JMJD2a polyclonal antibody
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Catalog number
6851-25
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Price
Please ask
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Size
25 μg
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Antibody Target
JMJD2a
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Target Alternative Name
JHDM3A, KDM4A, JMJD2
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Antibody Type
Polyclonal
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Species Reactivities
Human.
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Immunogen Sequence
Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
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Accession
O75164
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Gene Id
9682
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
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Purification
Affinity Purified
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Application
Western blot, ELISA.
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Positive Control
Western blot: HeLa cells, ELISA: Peptides.
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Application And Usages
Western Blot: 1:500, ELISA: 1:100 – 1:500.
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Description
JMJD2a belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
KDM4A
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Short name
JMJD2a polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Rabbit IgG
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Alternative name
JMJD2a polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene name
lysine demethylase 4A
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Synonyms gene
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Synonyms gene name
- jumonji domain containing 2
- jumonji domain containing 2A
- lysine (K)-specific demethylase 4A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2003-08-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tudor domain containing
- Lysine demethylases
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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