Human CellExp™ DPPII / QPP / DPP7, human recombinant

• Catalog number
  7512-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  DPPII, QPP, DPP7, DPP2, Dipeptidyl peptidase 2, Dipeptidyl aminopeptidase II
• Alternative_names
  DPPII, QPP, DPP7, DPP2, Dipeptidyl peptidase 2, Dipeptidyl aminopeptidase II
• Description
  ≥95% pure, active, human cell expressed, human recombinant DPPII / QPP / DPP7 - plays an important role in the degradation of some oligopeptides.
• Recombinant
  Yes
• Source
  HEK293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC (KP-AMC). The specific activity is >4000 pmol/min/µg.
• Results
  >4000 pmol/min/µg.
• Molecular Weight
  This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 53 kDa. The predicted N-terminus is Gly 22. DTT-reduced Protein migrates as 60-66 kDa due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
• Background Information
  Dipeptidyl peptidase 2 (DPP2 or DPP II) is also known as Dipeptidyl peptidase 7 (DPP7), Quiescent cell proline dipeptidase (QPP), which belongs to the peptidase S28 family. DPP7 / DPP2 is detected in seminal plasma (at protein level). When the pH is 5.5, Dipeptidyl peptidase 2 displays an optimum catalytic activity. DPP7 has low activity at pH 7 and is inactive at pH 8. DPP7 plays an important role in the degradation of some oligopeptides.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   DPPII   QPP   DPP7  
• Gene symbol
  DPP7
• Short name
  CellExp™ DPPII / QPP / DPP7, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ DPPII / QPP / DPP7, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:14892
• Gene
  DPP7
• Long gene name
  dipeptidyl peptidase 7
• Synonyms gene name
  • dipeptidylpeptidase 7
• Synonyms
  • DPPII
  • DPP2
• GenBank acession
  • AF154502
• Locus
  9q34.3
• Discovery year
  2001-04-20
• Entrez gene record
  29952
• Pubmed identfication
  • 10477574
  • 11139392
• RefSeq identity
  • NM_013379
• Classification
  • DASH family
• VEGA ID
  OTTHUMG00000020977

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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