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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr44 of human ENO1 (AA41-47)
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Antibody s target
Human ENO1 (pTyr44)
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Antibody s full description
Rabbit Anti-Human ENO1 (pTyr44) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
2-phospho-D-glycerate hydro-lyaseAntibody, Alpha enolase Antibody, C myc promoter binding protein Antibody, ENOA_HUMAN Antibody, Enolase 1 (alpha) Antibody, MBP 1 Antibody, MBPB1 Antibody, NNE Antibody, Non neural enolase Antibodyy, Plasminogen binding protein Antibody, PPH Antibody, Tau crystallin Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 47.169 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-965 was sufficient for detection of ENO1 (pTyr44) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus, Cytoplasm, Cell Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
ENO1 or Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is down-regulated in oxLDL-treated cells or in VLDL-treated cells. It is also identified to be associated with glucose metabolism being down-regulated in the process of foam cells formation.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001188412
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Gene number
2023
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Protein number
P06733
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
RPE, ENO1-AS1, ENO1-IT1, ENO1
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Short name
Rabbit Anti- ENO1 (pTyr44) Polyclonal: RPE
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
RPE
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman ENO1 (pTyr44) polyclonal: RPE
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Alternative technique
polyclonals, rabbit-anti, antibodies
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