SK-MEL-1 Cell Line
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Catalog numberEP-CL-0440
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationSK-MEL-1
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age29 years
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MorphologySpherical
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Growth_propertiesClusters In Suspension
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Doubling_timeNA
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BackgroundElectron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
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Biosafety1
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MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in nude mice forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters.
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Receptor_expressionNA
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Antigen_expressionBlood Type A; Rh＋
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Gene_expressionNA
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ApplicationsAntibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolDKK1, RAB38, ARMC9, TMOD4, RAB8A, MTNR1A, PCGF2, SSX2, CSPG4, IGHVII-1-1
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Short nameSK-MEL-1 Line
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SpeciesHomo sapiens, Human
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Alternative nameSK-MEL-1 cellular Line
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TissueMalignant melanoma, skin, derived from metastatic site: lymphatic system
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Gene info
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Identity
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Gene
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Long gene namedickkopf WNT signaling pathway inhibitor 1
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Synonyms gene name
- dickkopf (Xenopus laevis) homolog 1
- dickkopf 1 homolog (Xenopus laevis)
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Synonyms
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Locus
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Discovery year2000-09-01
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRAB38, member RAS oncogene family
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-16
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Entrez gene record
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Pubmed identfication
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namearmadillo repeat containing 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-10-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo repeat containing
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametropomodulin 4
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Synonyms gene name
- tropomodulin 4 (muscle)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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Classification
- Tropomodulins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRAB8A, member RAS oncogene family
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Synonyms gene
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Synonyms gene name
- mel transforming oncogene (derived from cell line NK14)
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Synonyms
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemelatonin receptor 1A
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Synonyms
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Locus
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Discovery year1995-03-22
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Entrez gene record
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Pubmed identfication
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Classification
- Melatonin receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolycomb group ring finger 2
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Synonyms gene
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Synonyms gene name
- ring finger protein 110
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polycomb group ring fingers
- Ring finger proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSSX family member 2
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Synonyms gene
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Synonyms gene name
- synovial sarcoma, X breakpoint 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-03-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SSX family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namechondroitin sulfate proteoglycan 4
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Synonyms gene name
- chondroitin sulfate proteoglycan 4 (melanoma-associated)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Proteoglycans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
