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Immunogen
Synthesized unphosphorylated peptide derived from human CD44 around the phosphorylation site of serine 706 (S-K-SP-Q-E).
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Antibody s target
Human CD44
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Antibody s full description
Rabbit Anti-Human CD44 Polyclonal
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Primary research fields
Immunology, Cell Type Markers, CD Markers, Stem Cells, Mesenchymal Stem Cells, MSC Surface Molecules, Stem Cells, Hematopoietic Progenitors, Lymphoid, T Lymphocytic Lineage, Stem Cells, Hematopoietic Progenitors, Myeloid, Dendritic Cell Lineage, Stem Cells, Hematopoietic Progenitors, Myeloid, Neutrophil Lineage
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
LHR Antibody, BA-1 Antibody, CD 44 Antibody, CDw44 Antibody, Cell surface glycoprotein CD44 Antibody, CSPG8 Antibody, ECMR-III Antibody, HCELL Antibody, HUTCH-I Antibody, MUTCH1 Antibody, PGP-1 Antibody, Phagocytic glycoprotein 1 Antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total CD44 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1271 was sufficient for detection of CD44 in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
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Scientific context
CD44 is a type I transmembrane glycoprotein involved in cell-cell interactions, cell adhesion and migration through its affinity for hyaluronic acid and possibly through other parts of the extracellular matrix. Its function is controlled by its posttranslational modifications. Clinically, it is significant as a cell surface marker for some breast and prostate cancer stem cells. It is also an indicative marker for effector-memory T-cells. CD44 has been shown to interact with Fibrin, Selectins and Src.
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Bibliography
1. Goodison S., et al. (1999) Mol. Pathol. 52: 189-196. 2. Cichy J. and Puré E. (2003) J. Cell Biol. 161: 839-843. 3. Li F., Tiede B., Massagué J., Kang Y. (2007). Cell Research. 17(1): 3–14. 4. Alves C.S., Burdick M.M., Thomas S.N., Pawar P., Konstantopoulos K. (2008). American Journal of Physiology. Cell Physiology. 294(4): C907–916. 5. Hanley W.D., Burdick M.M., Konstantopoulos K., Sackstein R. (2005). Cancer Research. 65(13): 5812–5817.
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Released date
6/Sep/2016
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NCBI number
NP_000601.3
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Gene number
960
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Protein number
P16070
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~81kDa CD44 protein using Rabbit Anti-CD44 Polyclonal Antibody (SPC-1271). Lane 1: Mouse NIH/3T3 cells, treated with PMA (250ng/ml, 5mins). Lane 2: Untreated. Primary Antibody: Rabbit Anti-CD44 Polyclonal Antibody (SPC-1271) at 1:1000. Predicted/Observed Size: ~81kDa. Rabbit Anti-CD44 Antibody used in Western blot (WB) on NIH/3T3 cell lysates (SPC-1271)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
CD44-AS1, CD44
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Short name
Rabbit Anti- CD44 Polyclonal: ATTO 488
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
ATTO 488
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman CD44 molecule (Indian blood group) polyclonal: ATTO 488
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
CD44 molecule (Indian blood group), CDW44 and CSPG8 and ECMR-III and HCELL and HUTCH-I and IN and LHR and MC56 and MDU2 and MDU3 and MIC4 and Pgp1, CD44 and IDBG-39556 and ENSG00000026508 and 960, hyaluronic acid binding, Cell surfaces, Cd44 and IDBG-193505 and ENSMUSG00000005087 and 12505, CD44 and IDBG-636564 and ENSBTAG00000011578 and 281057
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