GFP, Green Fluorescence Protein (affinity purified)

• Catalog number
  AS15 2987
• Price
  Please ask
• Size
  100 µg

• Available ordering format
  Inquire
• Immunogen
  highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at 4°C, or in small aliquots at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  ELISA (ELISA), Western blot (WB), Immunofluorescence (IF)
• Connected products
  AS15 3000 | GFP (protein A purified), rabbit antibodyAS15 2999 | GFP (total IgG), rabbit antibodyAS15 3001 | GFP, peroxidase conjugated, rabbit antibodyAS15 2998 | GFP (total IgY), chicken antibodyAS15 3002 | GFP (purified recombinant protein)other tag antibodies
• Recommended dilutions for use
  1: 5000 - 1: 25 000 (ELISA), 1: 500 (IF), 1: 2000 - 1: 10 000 (WB)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  native GFP, recombinant GFP (E.coli), all variants of GFP
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Minimal cross-reactivity with E.coli proteins.
• References
  to be added when available, antibody released in October 2015
• Scientific context
  GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
• Notes
  None
• Protein number
  P42212
• TAIR number
  Refer to NCBI
• Hyperlink
• Test
  A high affinity purification column was use to purify GFP, Protein ( ) by agisera by chromatographic size exclusion. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• Description
  1 of protein A or G purified by agisera will give more specificity than crude serum.

• Gene target
  GFP   Protein  
• Short name
  GFP, Protein ( )
• Technique
  affinity, purified, purifications
• Label
  gfp
• Alternative name
  Green Fluorescent, Green Fluorescence Protein (protein enriched)
• Alternative technique
  pure

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