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Rat Histone H4 ELISA Kit

Rat Histone H4 ELISA Kit is available 1 time from Sab elisa kit labs

EK3475 | Rat Histone H4 ELISA Kit size: 96-wells plate (breakable strips) | 692.28 USD

Catalog number EK3475
Price 692.28 USD
Size 96-wells plate (breakable strips)
1. Gene info
Identity 20510
Gene H4-16
Long gene name H4 histone 16
Synonyms gene
  • HIST4H4
Synonyms gene name
  • histone 4, H4
  • histone cluster 4, H4
  • histone cluster 4 H4
Synonyms
  • MGC24116
GenBank acession
  • AY128653
Locus 12p12.3
Discovery year 2003-02-20
Entrez gene record 121504
Pubmed identfication
  • 12408966
RefSeq identity
  • NM_175054
Classification
  • H4 histones
Havana BLAST/BLAT OTTHUMG00000168735
Product images
    #Rat#.png
Long name ELISA test kit for Rat Histone H4
Synonym name Rattus Histone H4 ELISA Kit
General description This is a ready to use kit for performing ELISA tests for detection of Histone H4 in samples from Rat
Antigen Histone H4
Species reactivity Rat
Sample types Our Histone H4 ELISA kit has been proven to work and successfully detect Histone H4 in Rat
Kit contents ELISA test kit for Rat Histone H4Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
Detection wavelengt 450 nm
Assay procedure 1-4h
Summary of the prodecure 1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
Applications Enzyme Linked Immunosorbent Assay
Shipping, storage, shelf life The Histone H4 ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Rat Histone H4 ELISA Kit is 6 months after delivery.
Tips Due to transportation and handling small amounts of the liquid components of the Rattus Histone H4 ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name Rattus norvegicus
Gene targetHistone
Short name Histone H4 ELISA
Technique ELISA kit, ELISA
Host Rat
Species Rat
Alternative name Rat Histone H4 Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Histone H4 ELISA | Technique alternative | 01014428493
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