-
Description
Dulbecco's Modified Eagle Medium (DMEM), With 4.5gm Glucose per litre Without L-Glutamine, Sodium bicarbonate and Sodium pyruvate, is an off-white to creamish white, homogenous powder. It was originally used to culture embryonic mouse cells. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines.
-
-
Shelf life
Use before expiry date given on the product label.
-
Storage
Store at 2-8°C away from bright light
-
Appearance
Off-white to Creamish white.
-
PH without Sodium Bicarbonate
6.00 -6.60
-
PH with Sodium Bicarbonate
7.50 -8.10
-
Osmolality without Sodium Bicarbonate
250.00 -290.00
-
Osmolality with Sodium Bicarbonate
330.00 -370.00
-
Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimumthree subcultures.
-
Solubility
Clear solution at 12.8 gms/L.
-
Uses
Laboratory chemicals, Manufacture of substances
-
Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
-
Toxicological Information
NA
-
-
-
Ordering
To order Dulbecco's Modified Eagle Medium (DMEM) With 4.5gm Glucose per litre Without L-Glutamine, Sodium bicarbonate and Sodium pyruvate, please use the Cat. Nr.AT066A-10X5L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
-
Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Dulbecco's Modified Eagle Medium (DMEM) With 4.5gm Glucose per litre Without L-Glutamine, Sodium bicarbonate and Sodium pyruvate
-
Additional description
The Liter or liter is a metric system unit of volume equal to 1 cubic decimeter (dm3), 1,000 cubic centimeters (cm3) or 1/1,000 cubic meter. A cubic decimeter (or liter) occupies a volume of 10×10×10 centimeters (see figure) and is thus equal to one-thousandth of a cubic meter. Water baths of various volumes are available for lab research. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
-
Group
media
