Pig Alpha-2-HS-glycoprotein ELISA Kit
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Catalog numberEK1441
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PricePlease ask
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Size96-wells plate (breakable strips)
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Long nameELISA test kit for Pig Alpha-2-HS-glycoprotein
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Synonym namePorcine Alpha-2-HS-glycoprotein ELISA Kit
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General descriptionThis is a ready to use kit for performing ELISA tests for detection of Alpha-2-HS-glycoprotein in samples from Pig
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AntigenAlpha-2-HS-glycoprotein
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Species reactivityPig
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Sample typesOur Alpha-2-HS-glycoprotein ELISA kit has been proven to work and successfully detect Alpha-2-HS-glycoprotein in Pig
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Kit contentsELISA test kit for Pig Alpha-2-HS-glycoproteinAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
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Detection wavelengt450 nm
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Assay procedure1-4h
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Summary of the prodecure1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
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ApplicationsEnzyme Linked Immunosorbent Assay
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Shipping storage shelf lifeThe Alpha-2-HS-glycoprotein ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Pig Alpha-2-HS-glycoprotein ELISA Kit is 6 months after delivery.
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TipsDue to transportation and handling small amounts of the liquid components of the Porcine Alpha-2-HS-glycoprotein ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
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DescriptionThe Alpha-2-HS-glycoprotein ELISA is a α- or alpha protein sometimes glycoprotein present in blood.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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AboutPigs and the smaller guinea pigs are frequent used as models for humans.
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Gene target
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Gene symbolTRADD, NRXN1, APPBP2, HSPB2, CLDN7, GIPC1, MAP3K14, BCS1L, HSPB1, NPRL3
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Short nameAlpha-2-HS-glycoprotein ELISA
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesPig, Pigs and Guinea pigs
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Alternative nameswine a-2-HS-glycoprotein Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameTNFRSF1A associated via death domain
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Synonyms gene name
- TNFRSF1A-associated via death domain
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-05-07
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameneurexin 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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RefSeq identity
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Classification
- Neurexins
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameamyloid beta precursor protein binding protein 2
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Synonyms gene name
- amyloid beta precursor protein (cytoplasmic tail)-binding protein 2
- amyloid beta precursor protein (cytoplasmic tail) binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 2
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Synonyms gene name
- heat shock 27kD protein 2
- heat shock 27kDa protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-23
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Entrez gene record
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Pubmed identfication
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Classification
- Small heat shock proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameclaudin 7
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-11-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Claudins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGIPC PDZ domain containing family member 1
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Synonyms gene
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Synonyms gene name
- chromosome 19 open reading frame 3
- regulator of G-protein signalling 19 interacting protein 1
- GIPC PDZ domain containing family, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-01
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Entrez gene record
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Pubmed identfication
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Classification
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitogen-activated protein kinase kinase kinase 14
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitogen-activated protein kinase kinase kinases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameBCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone
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Synonyms gene name
- BCS1 (yeast homolog)-like
- BCS1-like (yeast)
- BCS1-like (S. cerevisiae)
- BC1 (ubiquinol-cytochrome c reductase) synthesis-like
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial respiratory chain complex assembly factors
- AAA ATPases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 1
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Synonyms gene name
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-09
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Entrez gene record
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Pubmed identfication
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Classification
- Small heat shock proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNPR3 like, GATOR1 complex subunit
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 35
- nitrogen permease regulator-like 3 (S. cerevisiae)
- NPR3-like, GATOR1 complex subunit
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Synonyms
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Synonyms name
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Locus
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Discovery year2000-12-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GATOR1 subcomplex
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data