-
Category
Secondary Antibody
-
Antibody Subtype
Conjugated Secondary Antibodies
-
Area of research
Immunology
-
Type of Immunogen
Purified Mouse IgM (H&L)
-
Raised in
Goat
-
Specificity
This antibody reacts with heavy (mu) chains on mouse IgM and light chains on all mouse immunoglobulins.
-
Cross Reactivity
No reactivity is observed to non-immunoglobulin mouse serum proteins.
-
Conjugate Tag
Alkaline Phosphatase
-
Grade Purity
Grater than 95% pure
-
Method of Purification
Affinity purified using solid phase Mouse IgM
-
Source
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
-
Concentration
1.50 mg/ml (lot specific)
-
Form Buffer
Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
-
Storage
Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.
-
Shipping conditions
Blue Ice
-
Tested for
Please refer to the datasheet
-
Usage Recommendations
1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
-
Latin name
Capra aegagrus hircus, Mus musculus
-
Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Gene target
-
Short name
Goat anti Mouse IgM H + L Alk Phos
-
Technique
anti mouse, Mouse, anti, Goat, IgM, antibody to, anti mouse antibodies, goats, IgMs, mouses
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
IgM, IgM
-
Species
Mouse, Goats, Mouses
-
Alternative name
Goat anti Mouse IgM (H + L) secondary antibody (Alk Phos)
-
Alternative technique
murine, antibodies, caprine, immunoglobulin-m
-
Alternative to gene target
anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642
-