Conjugated Primary Antibody
ALEXA FLUOR® 594
Rabbit (Oryctolagus cuniculus)
This antibody reacts specifically with CPA3/MC-CPA
No modification has been applied to this antibody
1ug per 1ul
KLH conjugated synthetic peptide derived from human CPA3/MC-CPA
Applications with corresponding dilutions
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.
Purified by Protein A.
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
carboxypeptidase A; Carboxypeptidase A3; carboxypeptidase A3 mast cell; CBPA3_HUMAN; Cpa3; Mast cell carboxypeptidase A; mast cell carboxypeptidase A3; MC CPA; MC-CPA.
Also known as
CPA3/MC-CPA Polyclonal Antibody
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
For facs or microscopy Alexa 1 conjugate.
Alexa Fluor,ALEXA FLUOR® 594
Alexa conjugate 1
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Immunoglobulin G (IgG)
ALEXA FLUOR 594
CPA3/MC-CPA Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
Alternative to gene target
carboxypeptidase A3 (mast cell), MC-CPA, CPA3 and IDBG-60396 and ENSG00000163751 and 1359, zinc ion binding, Extracellular, Cpa3 and IDBG-133537 and ENSMUSG00000001865 and 12873, CPA3 and IDBG-637892 and ENSBTAG00000015008 and 528454