USP5 Antibody
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Catalog number6132-50
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PricePlease ask
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Size50 μg
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Antibody TargetUSP5
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Target Alternative NameDeubiquitinating enzyme 5, Isopeptidase T, IsoT, ISOT, Ubiquitin carboxyl-terminal hydrolase 5, Ubiquitin-specific-processing protease 5, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5.
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceRecombinant full length protein
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AccessionP45974
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Gene Id8078
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AppearanceColorless liquid
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FormLiquid
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Formulation50 µg of antibody in PBS containing 10% glycerol
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PurificationPurified from egg yolk
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ApplicationWestern blot
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Application And UsagesWestern blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
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DescriptionThe ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP5 (ubiquitin specific peptidase 5), also known as ISOT (isopeptidase T), is a 858 amino acid zinc-binding deubiquitinating enzyme that participates in the Ub pathway. A member of the peptidase C19 family, the catalytic activity of USP5 involves a combination of the ubiquitin carboxyl-terminal thiolester and water to produce ubiquitin and a thiol. USP5 contains two UBA domains and one UBP-type zinc finger. USP5 is responsible for disassembling unanchored polyubiquitin chains in the cell. Full length USP5 mediated cleavage of Ub-PLA2 is significantly enhanced is enhanced by low concentrations of mono ubiquitin.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolUSP5
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Short nameUSP5 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeChicken IgG
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Alternative nameUSP5 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameubiquitin specific peptidase 5
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Synonyms gene name
- ubiquitin specific peptidase 5 (isopeptidase T)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-02-01
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Pubmed identfication
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Classification
- Ubiquitin specific peptidases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data