Annexin V Apoptosis Detection Kit with 7AAD-FITC

• Catalog number
  ANXVKF7-100T
• Price
  Please ask
• Size
  100 TEST

• Description
  Annexin V Apoptosis Detection Kit with 7AAD-FITC is a kit used for the identifacation and detection of apoptotic and necrotic cells, this Kit was conjugated with FITC format and reported for use in flow cytometric analysis, it reacts with Annexin V proteins, with 7-amino-actinomycin D (7-AAD) helps to make the difference between apoptic cells and necrotic, used also as a dead cell exclusion dye.
• Other apoptosis tools
• Other name
  Annexin A5 Apoptosis Detection Kit
• Application
  Flow Cytometry Method
• Conjugate
  FITC
• Reactivity
  All mammalian
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
• Expiration
  After 1 year
• Storage
  2-8°C, store in dark, do not freeze
• Remark
  For Recherche Use Only.
• Additional description
  This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Immunostep has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies. The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
• Properties
  This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Annexin   Kit   with   7AAD  
• Short name
  Annexin V Apoptosis Kit with 7AAD-FITC
• Technique
  apoptosis, detection, FITC, apoptosis pathway, Fluorescein
• Label
  FITC
• Alternative name
  Annexin V programmed cellular destruction quantification reagent including 7AAD-fluorecein
• Alternative technique
  celldeath, fluorescine, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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