Mutant Alanine Racemase Y354N, Active, Recombinant

• Catalog number
  P1116-200
• Price
  Please ask
• Size
  200 ug

• Synonyms
  Alanine Racemase Y354N, alr, dal
• Alternative_names
  Alanine Racemase Y354N, alr, dal
• Description
  Catalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  > 99%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to convert 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
• Biological activity
  Specific activity is ≥ 50 mU/mg in which one unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
• Results
  ≥ 50 mU/mg
• Unit Definition
  One Unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate
• Molecular Weight
  43 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized form proprietary buffer
• Reconstitution Instructions
  Reconstitute enzyme in 50 mM phosphate buffer, pH 7.4.
• Background Information
  Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Mutant   Alanine   Racemase   Y354N   Active  
• Short name
  Mutant Alanine Racemase Y354N, Active, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Mutant Alanine Racemase Y354N, functionnal, Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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