Filters
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing
Ajax processing

Thrombin Sepharose Beads

Thrombin Sepharose Beads is available 3 times from Biovision labs

7925-25 | Thrombin Sepharose Beads size: 25 ml | 1,871.79 USD

Loading

7925-5 | Thrombin Sepharose Beads size: 5 ml | 757.58 USD

Catalog number 7925-5
Supplier Biovision
Price 757.58 USD
Size 5 ml
Product images
    Thrombin Sepharose Beads

7925-1 | Thrombin Sepharose Beads size: 1 ml | 257.58 USD

Loading
Description Beads for convenient cleavage and purification of recombinant fusion proteins containing thrombin-specific cleavage sites.. Thrombin Sepharose beads are designed for efficient cleavage of recombinant fusion proteins, containing a thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site), circumventing the need for chromatographic techniques to remove thrombin after cleavage reaction is completed. Thrombin Sepharose is prepared by covalent coupling of bovine thrombin to activated 6% cross-linked Sepharose beads. 15 µl of the slurry is sufficient to cleave >90% of 1 mg of 6xHis-tagged fusion protein in 50 mM Tris buffer, 0.1 M NaCl, pH 8.0 within 4 h at room temperature. These beads can be regenerated for repeat use. The reusability depends on multiple criteria including the usage and handling of the product.
Product Highlights FORMULATION: 6% cross linked Sepharose beads provided as 50% slurry in pure glycerol. LIGAND DENSITY: 1 mg/ml of the resin. APPLICATIONS: Efficient and convenient cleavage of recombinant fusion proteins containing thrombin-specific cleavage site. PROTOCOL: In order to find the optimum cleavage conditions for a target fusion protein, it is recommended to run preliminary cleavage reactions at a small scale. Successful cleavage with thrombin is dependent upon proper folding of the fusion protein that enables access of the thrombin recognition sequence by the enzyme. Once optimum cleavage conditions are obtained, the reaction can be scaled up to cleave the entire amount of the target fusion protein. The target fusion protein should be purified to homogeneity and dialyzed against 50 mM Tris buffer, 0.1 M NaCl, pH 8.0 before setting up the cleavage reaction. 1) Resuspend the beads by gentle swirling. Do not Vortex. 2) Aliquot 15 µl of the suspended slurry and add to 1 mg of the fusion protein in an Eppendorf tube. The recommended concentration of target fusion protein is 1 mg/ml. 3) Mix gently by inverting the tube (do not vortex) and gently shake on a rotary shaker at room temperature. 4) At regular time intervals spin down the tube to aliquot a test sample and freeze it immediately. At the end of the reaction, analyze the samples by SDS-PAGE. Recovery of the cleaved target protein: 1) After the fusion protein is completely cleaved, spin down the reaction mixture for 2-3 min at 5000 rpm. 2) Remove the supernatant and wash the beads with 0.2 ml of 50 mM Tris buffer, pH 8. 3) Repeat steps 1 and 2 to maximize the recovery of the target protein and its cleaved fragment. Further chromatography may be necessary to remove the cleaved fragments from the target protein.
Storage Temp. -20°C
Shipping gel pack
Shelf Life 12 months
Notes For research use only. Not to be used for human or animal treatment or consumption.
Gene targetThrombin Sepharose
Short name Thrombin Sepharose
Technique All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet.
Label beads
Alternative name Thrombin Sepharose particles
Similar products
Human Melanoma- associated antigen B10, MAGEB10 ELISA KIT Suppplier: Lifescience Market
Price: 960.37 USD
Rat RUNX2 Runt Related Transcription Factor 2 Kit Suppplier: Elabscience
Price: 573.43 USD
SSBP1 siRNA (Rat) Suppplier: MyBioSource
Price: 527.97 USD
COX5B Antibody Suppplier: Assay Biotech
Price: 378.79 USD
Buffer P3 Suppplier: GeneAll
Price: 377.62 USD
Antibody GST Suppplier: Kamiya
Price: 332.17 USD
SBI 0640756 Suppplier: MedChemExpress
Price: 314.69 USD
LentimiRa Off hsa miR 6736 Suppplier: ABM microrna
Price: 294.87 USD
LentimiRa rno mir 344i Suppplier: ABM microrna
Price: 294.87 USD
Rabbit Anti Human GATA5 Antibody Suppplier: Cusabio
Price: 247.09 USD
G6PD 1 491aa E Coli E Coli Suppplier: MyBioSource
Price: 5.83 USD
Mycoplasma pneumoniae Uncharacterized protein MPN_581 MPN_581 Suppplier: MBS Recombinant
Price: 2 073.42 USD
Salmonella paratyphi A Acetylglutamate kinase argB Suppplier: MBS Recombinant
Price: 2 054.78 USD
Mouse Pi4k2b ELISA KIT Suppplier: Lifescience Market
Price: 1 008.16 USD
RPS27P19 3 UTR Suppplier: ABM microrna
Price: 1 434.73 USD
Ccdc136 3 UTR Luciferase Stable Cell Line Suppplier: ABM microrna
Price: 0.00 USD
Ask
Ajax processing
Close
Thrombin Sepharose | Technique alternative | 01012192260
Filters
EU:+32-(0)1-658-90-45 US:+1-(408)780-0908 [email protected]
  • Thrombin
  • Sepharose
Back
Contact us
Ajax processing
Close
Chat with gentaur.com employee