Rabbit Anti-Rat NCC Polyclonal: ATTO 700

  • Catalog number
    SPC-402D-A700
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Poduced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
  • Antibody s target
    Rat NCC
  • Antibody s full description
    Rabbit Anti-Rat NCC Polyclonal
  • Primary research fields
    Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody
  • Verified applications
    WB, IHC, ICC/IF, IEM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat, Dog
  • Antibody s recommended dilutions for use
    WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~160kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
  • Bibliography
    1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
  • Released date
    11/Jun/2010
  • NCBI number
    NP_062218, NP_000330
  • Gene number
    54300, 6559
  • Protein number
    P55018, P55017
  • PubMed number
    27733368|27440776|27206969|26582762|26239621|25941340|25492393|24942911|24668812|24668812|23482560|23482560|22966159
  • Tested applications
    |Western Blot|Immunohistochemistry||Western Blot|Western Blot|Western Blot|Western Blot|Electron Microscopy|Western Blot|Western Blot|Western Blot|Immunohistochemistry
  • Tested reactivity
    |Rat|Mouse|Mouse|Mouse|Mouse|Rat|Human|Dog|Dog|Dog|Human|Rat
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000. Rabbit Anti-NCC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-402) | Rabbit Anti-NCC Antibody used in Western blot (WB) on Rat tissue lysates (SPC-402)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Oryctolagus cuniculus, Rattus norvegicus
  • Gene target
    NCC   ATTO   700  
  • Gene symbol
    CCL13, CCL14, CCL15, CCL16
  • Short name
    Rabbit Anti- NCC Polyclonal: ATTO 700
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    ATTO 700
  • Species
    Rat, Rats
  • Alternative name
    production species: rabbit Antibody toRat NCC polyclonal: ATTO 700
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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