Rabbit Anti-Rat NCC Polyclonal: ATTO 700
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Catalog numberSPC-402D-A700
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PricePlease ask
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Size100 µg
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ImmunogenPoduced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
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Antibody s targetRat NCC
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Antibody s full descriptionRabbit Anti-Rat NCC Polyclonal
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Primary research fieldsCancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameSLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody
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Verified applicationsWB, IHC, ICC/IF, IEM
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Raised inRabbit
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Antibody s reactivityHuman, Mouse, Rat, Dog
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Antibody s recommended dilutions for useWB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromProtein A Purified
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Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~160kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysis1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service.
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Scientific contextNCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
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Bibliography1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
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Released date11/Jun/2010
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NCBI numberNP_062218, NP_000330
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Gene number54300, 6559
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Protein numberP55018, P55017
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PubMed number27733368|27440776|27206969|26582762|26239621|25941340|25492393|24942911|24668812|24668812|23482560|23482560|22966159
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Tested applications|Western Blot|Immunohistochemistry||Western Blot|Western Blot|Western Blot|Western Blot|Electron Microscopy|Western Blot|Western Blot|Western Blot|Immunohistochemistry
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Tested reactivity|Rat|Mouse|Mouse|Mouse|Mouse|Rat|Human|Dog|Dog|Dog|Human|Rat
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000. Rabbit Anti-NCC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-402) | Rabbit Anti-NCC Antibody used in Western blot (WB) on Rat tissue lysates (SPC-402)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameOryctolagus cuniculus, Rattus norvegicus
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Gene target
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Gene symbolCCL13, CCL14, CCL15, CCL16
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Short nameRabbit Anti- NCC Polyclonal: ATTO 700
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TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelATTO 700
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SpeciesRat, Rats
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Alternative nameproduction species: rabbit Antibody toRat NCC polyclonal: ATTO 700
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 13
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 13
- chemokine (C-C motif) ligand 13
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 14
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 14
- chemokine (C-C motif) ligand 14
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 15
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 15
- chemokine (C-C motif) ligand 15
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 16
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 16
- chemokine (C-C motif) ligand 16
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data