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Category
Primary Antibodies
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Long description
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. Recently, it has been shown that cells which do not normally respond to NGF only express low affinity receptors from transfected NGF genes but cells which normally are NGF-responsive express high affinity receptors from the same NGF sequences. This supports the hypothesis that the high affinity receptors have a unique component in addition to the subunit common to the low affinity receptors.
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Antibody come from
Human recombinant nerve growth factor (hNGF).
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Other description
Provided as 0.2 µm sterile filtered solution in phosphate buffered saline with 0.1% sodium azide.
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Clone
AS22
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Antigen antibody binding interaction
Mouse anti Nerve Growth Factor (NGF) (Neutralizing) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Mouse, Bovine, Viper
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Enzyme Immunoassay
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Antibody s suited for
Applications: ELISA: This antibody has been used as a coating antibody at 10 µg/ml in a two site ELISA with biotin conjugated NGF antibody (Cat. No. L147M) for mouse NGF. Typically for mouse NGF, the ELISA has a working range of 0 -15 ng/ml and a sensitivity of 15 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human NGF in human plasma is typically > 90% in the ELISA. The use of Tris buffer, pH 7, is recommended. (Kenney, JS, Masada, MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990)._x000B__x000B_Neutralization: This antibody has been used to neutralize > 90% human NGF bioactivity on PC12 cells at a 200-fold molar excess.
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Storage
-20ºC
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Relevant references
Kenney, JS, Masada, MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. To cause (an acid or base) to undergo neutralization.
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Latin name
Mus musculus
