M-MuLV Reverse Transcriptase, 200U/ul

Storage Temperature

M-MuLV Reverse Transcriptase, 200U/ul should be stored at -20°C
Shipping Conditions

Ice pack
Retest

2 years
Application

For research use only. Not for diagnostic procedures, drug use, or for administration to humans or animals.
Hazardous

NA
Ordering

To order M-MuLV Reverse Transcriptase, 200U/ul , please use the Cat. Nr. A1111-5000U and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Tips

Our specialists recommend you to follow carefully the pre-registered instructions for M-MuLV Reverse Transcriptase, 200U/ul
Description

Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup

reverse transcription

Name

Reverse Transcriptase Polymerase Chain Reaction
Concept

Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Tree numbers
- E05.393.620.500.725
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data