Human Recombinant PIN1
Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, NIMA-interacting protein 1, DOD, UBL5, Rotamase Pin1, PPIase Pin1.
Plays a role as a post phosphorylation control in regulating protein function
Purity by SDS-PAGE
Endotoxin Level< 1.0 EU per 1 ug of protein (determined by LAL method)
> 330 nmoles/min/mg
Specific activity is defined as the amount of enzyme that cleaves 1 µmole of SucAAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin
18.2 kDa (163 aa)
Physical form description
1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5 mM DTT, 20% glycerol.
Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like β-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Amino acid sequence
MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Recombinants or rec. proteins
H. sapiens recombinant peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Alternative to gene target
peptidylprolyl cis/trans isomerase, NIMA-interacting 1, DOD and UBL5, PIN1 and IDBG-26087 and ENSG00000127445 and 5300, phosphothreonine binding, nuclei, PIN1 and IDBG-643081 and ENSBTAG00000016988 and 535470