Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

• Catalog number
  K828-100
• Price
  Please ask
• Size
  100 assays

• Description
  Market's first and only kit for screening Hexokinase Inhibitors. Simple and HTP compatible. Screen within 30 min.
• Summary
  A simple and high-throughput adaptable assay kit used to colorimetrically (450 nm) screen/study/characterize potential inhibitors of Hexokinase within 30 min.
• Detection Method
  Absorbance at 450 nm
• Species Reactivity
  N/A
• Applications
  • Screening/characterizing/studying potential inhibitors of Hexokinase
• Sample Type
  Variable: Inhibitors, small molecules, proteins, etc.
• Features Benefits
  Simple and High throughput adaptable Colorimetric Detection Screen within 30 min
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Hexokinases (HK) play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various tumors and is associated with metastasis. In BioVision’s Hexokinase Inhibitor Screening kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance. This assay kit can be used to screen/study/characterize the potential inhibitors of Hexokinase. The assay is simple, high-throughput adaptable and can be performed within 30 min.
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Hexokinase   Inhibitor   Screening   Kit  
• Short name
  Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Alternative name
  Hexokinase (HK) suppressor detection reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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