Human CellExp™ PCSK9, murine recombinant

• Catalog number
  7266-20
• Price
  Please ask
• Size
  20 ug

• Synonyms
  PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
• Alternative_names
  PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
• Description
  Plays a major regulatory role in cholesterol homeostasis
• Recombinant
  Yes
• Source
  HEK 293 cells
• Purity by SDS PAGE
  ≥97%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 ug/ml.
• Biological activity
  Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 ug/ml.
• Molecular Weight
  This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 72 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 20 kDa and 64 kDa due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Background Information
  Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   PCSK9   murine  
• Gene symbol
  PCSK9
• Short name
  CellExp™ PCSK9, murine recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ proprotein convertase subtilisin/kexin classification 9, murine Rec.
• Alternative technique
  rec
• Alternative to gene target
  proprotein convertase subtilisin/kexin type 9, PCSK9 and IDBG-99082 and ENSG00000169174 and 255738, very-low-density lipoprotein particle receptor binding, Extracellular, Pcsk9 and IDBG-170186 and ENSMUSG00000044254 and 100102

Gene info

• Identity
  HGNC:20001
• Gene
  PCSK9
• Long gene name
  proprotein convertase subtilisin/kexin type 9
• Synonyms gene
  • HCHOLA3
• Synonyms gene name
  • hypercholesterolemia, autosomal dominant 3
• Synonyms
  • NARC-1
  • FH3
• GenBank acession
  • AX207686
• Locus
  1p32.3
• Discovery year
  2003-05-13
• Entrez gene record
  255738
• Pubmed identfication
  • 12552133
  • 12730697
• RefSeq identity
  • NM_174936
• Classification
  • Proprotein convertase subtilisin/kexin family
• VEGA ID
  OTTHUMG00000008136
• Locus Specific Databases
  • British Heart Foundation
  • LRG_275

MeSH Data

• Name
  Urine Specimen Collection
• Concept
  Scope note: Methods or procedures used to obtain samples of URINE.
• Tree numbers
  • E01.370.225.998.762
  • E05.200.998.762
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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