Basic Exo-Flow kit without any coupled antibodies (Uncoupled magnetic streptavidin beads, Wash and Elution Buffers, Exo-FITC stain)

• Catalog number
  CSFLOWBASICA-1
• Price
  Please ask
• Size
  100 reactions

• Shipping Temperature
  Blue Ice
• Product category
  Exosomes
• Description
  Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
• Properties
  This Systembio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Systembio FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody, Magnetic beads
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

• Gene target
  Exo   kit   without   any   coupled   antibodies   Uncoupled   magnetic   streptavidin   Elution   Buffers   Exo   stain  
• Short name
  Exo- kit without any coupled antibodies (Uncoupled magnetic streptavidin , Elution Buffers, Exo-FITC stain)
• Technique
  FITC, Flow, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Fluorescein
• Label
  Magnetic
• Alternative name
  Basic Exo-Flow reagent without any coupled antibodies (Uncoupled magnetic biotinilated-binding streptavidin particles, washing and Elution Buffers, Exo-fluorecein staining)
• Alternative technique
  fluorescine, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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