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Stock availability
In Stock
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Scientific context
Aha2 shares 45% sequence homology with Aha1, however, little is known about the functional protein. Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
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Protein target
AHA2
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Protein reactivity
Human
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Certificate of analysis
This product has been certified >90% pure using SDS - PAGE analysis.
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Protein description
Human Recombinant AHA2 Protein
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Other name
ATPase hydrogen-exporting ATPase 2 Protein
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Primary research area
Cancer, Heat Shock
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Category
Protein
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Brand name
none
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Origin
Recombinant
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NCBI number
NP_036243.1
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Gene number
130872
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Protein number
Q719I0
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Verified applications
WB, SDS-PAGE
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Relevant bio activity
AHA2 Protein
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Protein expression model
E. coli
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Protein charasterics
See included datasheet.
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Peptide sequence
See included datasheet.
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Protein purification
Affinity Purified
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Purity pourcentage
>90% High purity
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Recommended buffer for storage
100mM NaH2 pH4.5, 10mM Tris buffer with 8M urea
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Protein concentration
Lot/batch specific. See included datasheet.
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Protein specificity
~38 kDa
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Protein tag
His tag
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Supplementary useful information
Please see included datasheet or contact us
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Bibliography
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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Release date
1-Aug-2011
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PubMed number
Not added. Please refer to PubMed
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Tested applications
to be tested
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Tested species reactivity
to be tested
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Representative figure legend
SDS-PAGE of ~38kDa his-tagged human Aha2 protein (SPR-312). SDS-Page of human AHA2 Protein (SPR-312)
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Warnings
Non-hazardous materials
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Protein origin
Canada
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Total weight kg
1.4
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Net weight g
0.05
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants