Mouse anti phosphotyrosine conjugated to rhodamine

  • Catalog number
    X1020
  • Price
    Please ask
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The rhodamine conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
  • Antibody come from
    Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
  • Other description
    Provided at a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 3mM sodium azide, 50% glycerol.
  • Clone
    PY20
  • Antigen antibody binding interaction
    Mouse anti phosphotyrosine conjugated to rhodamine Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Ubituitous
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot, Immunoprecipitation, Enzyme Immunoassay, Immunohistochemistry
  • Antibody s suited for
    Application: A dilution of 5-10 ug/ml is suggested for immunohistochemistry or immunocyto-chemistry.
  • Storage
    -20ºC
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse anti phosphotyrosine conjugated rhodamine
  • Technique
    Mouse, anti, antibody to, antibody Conjugates, mouses
  • Host
    mouse
  • Isotype
    IgG2b
  • Label
    Rhodamine
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to phosphotyrosine coupled to rhodamine
  • Alternative technique
    murine, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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