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25 Hydroxyvitamin D3 HVD3

25 Hydroxyvitamin D3 HVD3 is available 15 times from Bioma labs

EKU02021 | 25-Hydroxyvitamin D3 (HVD3) ELISA Kit, 96 well plate size: 5x96T | 4652.77 USD

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EKU02021 | 25-Hydroxyvitamin D3 (HVD3) ELISA Kit, 96 well plate size: 10x96T | 8411.31 USD

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EKU02021 | 25-Hydroxyvitamin D3 (HVD3) ELISA Kit, 96 well plate size: 96T | 1101.13 USD

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CAU29638 | 25-Hydroxyvitamin D3 (HVD3) Monoclonal Antibody size: 200 μg | 545.97 USD

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CAU29638 | 25-Hydroxyvitamin D3 (HVD3) Monoclonal Antibody size: 10 μg | 159.77 USD

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CAU29638 | 25-Hydroxyvitamin D3 (HVD3) Monoclonal Antibody size: 50 μg | 302.29 USD

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CAU29638 | 25-Hydroxyvitamin D3 (HVD3) Monoclonal Antibody size: 100 μg | 399.99 USD

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CAU29077 | 25-Hydroxyvitamin D3 (HVD3) Polyclonal Antibody size: 10 μg | 178.16 USD

Catalog number CAU29077
Supplier bioma
Price178.16 USD
Size 10 μg
Immunogen OVA Conjμgated 25-Hydroxyvitamin D3 (HVD3)
Verified reactivity Pan-species (General)
Verified applications WB, ICC, IHC-P, IHC-F, ELISA
Raised in Rabbit
Clonality Polyclonal
Protein number Please see Uniprot
Antibody's concentration 200μg/ml
Storage recommendation Aliquot and store at -20°C. Minimize freezing and thawing.
Properties If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation anticorps
Short name 25-Hydroxyvitamin D3 (HVD3) Polyclonal Antibody
Technique Polyclonal, Antibody
Alternative name 25-Hydroxyvitamin D3 (HVD3) polyclonal (Antibody to)
Alternative technique polyclonals
1. Gene info
Identity33013
Long gene name small nucleolar RNA, C/D box 114-25
Synonyms
Discovery year 2006-09-26
Entrez gene record767605
Pubmed identfication
RefSeq identity
Classification
  • Small nucleolar RNAs, C/D box
MeSH Data
Concept
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Additional information

CAU29077 | 25-Hydroxyvitamin D3 (HVD3) Polyclonal Antibody size: 200 μg | 705.73 USD

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CAU29077 | 25-Hydroxyvitamin D3 (HVD3) Polyclonal Antibody size: 100 μg | 462.06 USD

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CAU29077 | 25-Hydroxyvitamin D3 (HVD3) Polyclonal Antibody size: 50 μg | 341.37 USD

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RPU50526 | 25-Hydroxyvitamin D3 (HVD3), BSA Conjugated size: 50 μg | 417.23 USD

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RPU50526 | 25-Hydroxyvitamin D3 (HVD3), BSA Conjugated size: 200 μg | 745.96 USD

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RPU50526 | 25-Hydroxyvitamin D3 (HVD3), BSA Conjugated size: 100 μg | 608.03 USD

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RPU50526 | 25-Hydroxyvitamin D3 (HVD3), BSA Conjugated size: 10 μg | 265.51 USD

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Assay class Competitive
Other name 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Protein origin Peptide Synthesis
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Purity Affinity purified. Please contact us for more details
Samples to be analyzed Serum, plasma and other biological fluids
Estimated production time 7-11 business days
NCBI number Please refer to NCBI
Sensitivity limit 0,89 ng/mL
Alternate gene name 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
Gene number Please refer to GenBank
Assay duration 2 hours
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Protein sequence Please contact us.
Detection limits 2.47-200 ng/mL
Protocol Please see ELISA's datasheet, otherwise contact us
Stroage recommendation Aliquot and store at -20°C. Minimize freezing and thawing.
Notes For research use only. Not for diagnostic procedures.
Use for 8 months
Use before 1 year
Information about sequence Please contact us.
Research main area Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Shipping requirements Blue ice
ELISA detection Colorimetric
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of 25-Hydroxyvitamin D3 (HVD3). No significant cross-reactivity or interference between 25-Hydroxyvitamin D3 (HVD3) and analogues was observed.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 25-Hydroxyvitamin D3 (HVD3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 25-Hydroxyvitamin D3 (HVD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Alternate protein number Please refer to SwissProt
Protein purity ≥ 90%
Protein region Please contact us.
Assay principle This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 25-Hydroxyvitamin D3 (HVD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 25-Hydroxyvitamin D3 (HVD3) and unlabeled 25-Hydroxyvitamin D3 (HVD3) (Standards or samples) with the pre-coated antibody specific to 25-Hydroxyvitamin D3 (HVD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample.
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Supplementary information Applicable secondary antibody is available. Please inquire
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