3D Cell Culture Non-Enzymatic Cell Harvesting Kit

• Catalog number
  K982-100
• Price
  Please ask
• Size
  100 samples

• Summary
  • Detection method - Qualitative • Applications: Matrix and cell/spheroid dissociations • Recommended for experiment where cells are sensitive to protease digestion
• Detection Method
  Detection method - Qualitative
• Applications
  Matrix and Cell/Spheroid Dissociations Cell-harvesting for usage in cell-based assay, biochemical, and protein analysis Recommended for experiment where cells are sensitive to protease digestion.
• Sample Type
  3D Matrices • Adherent and suspension spheroids and cells
• Features Benefits
  • Easy, Fast, Reproducible, Qualitative tool for screening, studying, and characterizing compounds in cell culture studies • Recommended for experiment where cells are sensitive to protease digestion
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. However, when it comes to cell passaging, or studying biochemical processes and protein analysis, there are challenges with separating intact cells from extra-cellular proteins comprising the 3D matrices. While proteases (such as trypsin and accutase) are commonly used to degrade these matrices, it is not recommended in some cases where cells are sensitive to protease digestion; these proteases may modify cellular proteins and signaling or cell surface, thus altering physiological assessments. Further, some proteases-based dissociation methods do not completely dissolve the matrices. BioVision’s 3D Culture Non-Enzymatic Cell Harvesting Kit provides an optimized and standardized saline-based solution for the isolation of cells and spheroids from matrices (especially for BioVision’s 3D Cell Culture Kits: K517, 518, 519) with high viability rate for subsequent biochemical, protein and cell-based analysis.
• Data Sheet Link
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Culture   Non-Enzymatic   Harvesting   Kit  
• Short name
  3D Culture Non-Enzymatic Harvesting Kit
• Technique
  culture
• Alternative name
  3D cellular Culture Non-Enzymatic cellular Harvesting reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

MeSH Data

• Name
  Culture Techniques
• Concept
  Scope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
• Tree numbers
  • E05.481.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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