SMURF2 Antibody, ALEXA FLUOR 647
-
Catalog number
bs-4056R-A647
-
Price
Please ask
-
Size
100ul
-
-
Long name
SMURF2 Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
-
Also known as
Anti-SMURF2 PAb ALEXA FLUOR 647
-
Category
Conjugated Primary Antibodies
-
Conjugated with
ALEXA FLUOR® 647
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
SMURF2
-
Specificity
This is a highly specific antibody against SMURF2.
-
Modification
Unmodified
-
Modification Site
None
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen range
615-665/748
-
Subcellular location
Nucleus, Intracellular
-
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SMURF2
-
Gene ID Number
64750
-
Swiss Prot
Q9HAU4
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Background of the antigen
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
-
Purification
Purified by Protein A.
-
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
-
Excitation emission
650nm/665nm
-
Synonyms
E3 ubiquitin-protein ligase SMURF2; hSMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2
-
Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-SMURF2 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Conjugation
Alexa Fluor, ALEXA FLUOR 647
-
French translation
anticorps
-
Gene target
-
Gene symbol
SMURF2
-
Short name
Anti-SMURF2
-
Technique
Antibody, antibodies against human proteins, antibodies for
-
Isotype
Immunoglobulin G (IgG)
-
Label
ALEXA FLUOR 647
-
Alternative name
SMURF2 Polyclonal Antibody, ALEXA FLUOR 647
-
Alternative technique
antibodies
-
Alternative to gene target
SMAD specific E3 ubiquitin protein ligase 2, SMURF2 and IDBG-64876 and ENSG00000108854 and 64750, SMAD binding, nuclei, Smurf2 and IDBG-213588 and ENSMUSG00000018363 and 66313, SMURF2 and IDBG-641903 and ENSBTAG00000019853 and 540826
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
