Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Caspase-12 Staining Kit provides a convenient means for sensitive detection of activated caspase-12 in living cells. The assay utilizes the caspase-12 inhibitor, ATAD-FMK, conjugated to FITC (FITC-ATAD-FMK) as a marker. FITC-ATAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-12 in apoptotic cells.
• Detection method: Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Sensitive detection of activated caspase:12 in living cells.
• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The FITC label allows for direct detection of activated caspases in apoptotic cells
• FITC-ATAD-FMK • Wash Buffer • Z-VAD-FMK
Sensitive detection of activated caspase:12 in living cells.