PIP2;5, aquaporin, plasma membrane intrinistic protein 2-5
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Catalog numberAS09 508
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PricePlease ask
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Size100 µl
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Available ordering formatInquire
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ImmunogenKLH-conjugated peptide derived from Oryza sativa PIP2-5 Q8GRI8
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applicationsELISA (ELISA), western blot (WB)
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Connected productscollection of antibodies to plasma membrane proteins AS09 504 | anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3AS09 505 | anti-PIP1s | aquaporinAS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
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Molecular weight expected аpparent29.7 | 26-29 kDa
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Verified reactivityOryza sativa
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Possible reactivitymonocots including: Sorghum bicolor, Zea mays
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No reactivityArabidopsis thaliana
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
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ReferencesSakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
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Scientific contextPIP2;5 protein is an aquaporins which facilitates the transport of water and small neutral molecules across cell membrane.
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Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plasma membrane can be found here
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Protein numberQ8GRI8
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TAIR numberRefer to NCBI
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DescriptionAssociated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
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Gene target
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Gene symbolIGHVIII-5-2, TRI-AAT5-5, TRG-TCC2-5, TRN-GTT2-5, TRD-GTC2-5, TRY-GTA5-2, TRK-CTT2-5, TRM-CAT5-2, CYB5AP4, SFRP2
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Short namePIP2 5, aquaporin, plasma intrinistic protein 2-5
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Alternative namePIP2;5, aquaporin, plasma membrane intrinistic protein 2-5
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-5-2 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-5-2
- immunoglobulin heavy variable (III)-5-2 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon AAT) 5-5
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 23 (anticodon AAU)
- transfer RNA-Ile (AAT) 5-2
- transfer RNA-Ile (AAT) 5-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon TCC) 2-5
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 9 (anticodon UCC)
- transfer RNA-Gly (TCC) 2-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Asn (anticodon GTT) 2-5
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Synonyms gene
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Synonyms gene name
- transfer RNA asparagine 17 (anticodon GUU)
- transfer RNA-Asn (GTT) 2-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Asp (anticodon GTC) 2-5
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Synonyms gene
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Synonyms gene name
- transfer RNA aspartic acid 5 (anticodon GUC)
- transfer RNA-Asp (GTC) 2-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Tyr (anticodon GTA) 5-2
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Synonyms gene
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Synonyms gene name
- transfer RNA tyrosine 6 (anticodon GUA)
- transfer RNA-Tyr (GTA) 5-5
- transfer RNA-Tyr (GTA) 5-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Lys (anticodon CTT) 2-5
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Synonyms gene
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Synonyms gene name
- transfer RNA lysine 26 (anticodon CUU)
- transfer RNA-Lys (CTT) 2-2
- transfer RNA-Lys (CTT) 2-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Met (anticodon CAT) 5-2
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Synonyms
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Locus
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Discovery year2019-12-06
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namecytochrome b5 type A pseudogene 4
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Synonyms gene
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Synonyms gene name
- cytochrome b-5 pseudogene 4
- cytochrome b5 type A (microsomal) pseudogene 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-07-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesecreted frizzled related protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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Classification
- Secreted frizzled-related proteins
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VEGA ID