PIP2;5, aquaporin, plasma membrane intrinistic protein 2-5

  • Catalog number
    AS09 508
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Inquire
  • Immunogen
    KLH-conjugated peptide derived from Oryza sativa PIP2-5 Q8GRI8
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
  • Verified applications
    ELISA (ELISA), western blot (WB)
  • Connected products
    collection of antibodies to plasma membrane proteins AS09 504 | anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3AS09 505 | anti-PIP1s | aquaporinAS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1Plant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
  • Molecular weight expected аpparent
    29.7 | 26-29 kDa
  • Verified reactivity
    Oryza sativa
  • Possible reactivity
    monocots including: Sorghum bicolor, Zea mays
  • No reactivity
    Arabidopsis thaliana
  • Supplementary information
    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
  • References
    Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
  • Scientific context
    PIP2;5 protein is an aquaporins which facilitates the transport of water and small neutral molecules across cell membrane.
  • Notes
    0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plasma membrane can be found here
  • Protein number
    Q8GRI8
  • TAIR number
    Refer to NCBI
  • Description
    Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 
  • Gene target
  • Gene symbol
    IGHVIII-5-2, TRI-AAT5-5, TRG-TCC2-5, TRN-GTT2-5, TRD-GTC2-5, TRY-GTA5-2, TRK-CTT2-5, TRM-CAT5-2, CYB5AP4, SFRP2
  • Short name
    PIP2 5, aquaporin, plasma intrinistic protein 2-5
  • Alternative name
    PIP2;5, aquaporin, plasma membrane intrinistic protein 2-5
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-5-2 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-5-2
    • immunoglobulin heavy variable (III)-5-2 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Ile (anticodon AAT) 5-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA isoleucine 23 (anticodon AAU)
    • transfer RNA-Ile (AAT) 5-2
    • transfer RNA-Ile (AAT) 5-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Gly (anticodon TCC) 2-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA glycine 9 (anticodon UCC)
    • transfer RNA-Gly (TCC) 2-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Asn (anticodon GTT) 2-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA asparagine 17 (anticodon GUU)
    • transfer RNA-Asn (GTT) 2-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Asp (anticodon GTC) 2-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA aspartic acid 5 (anticodon GUC)
    • transfer RNA-Asp (GTC) 2-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Tyr (anticodon GTA) 5-2
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA tyrosine 6 (anticodon GUA)
    • transfer RNA-Tyr (GTA) 5-5
    • transfer RNA-Tyr (GTA) 5-2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Lys (anticodon CTT) 2-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA lysine 26 (anticodon CUU)
    • transfer RNA-Lys (CTT) 2-2
    • transfer RNA-Lys (CTT) 2-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
Gene info
Gene info
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