This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, ELISA
Target of this antibody:
Formulation of this antibody:
0.5 ml in 0.01 M sodium phosphate, 0.15 M NaCl, 1 mg/ml BSA, 25% glycerol, and 0.01% thimerosal.
Alternative names of antibody target:
Anti-Mouse IgG, HRP-Linked Antibody, HRP linked secondary antibody
Western blotting: 1:2,000-1:100,000. Enzyme immunoassays: 1:2,000-1:100,000. Immunohistochemistry: 1:200-1:2,000. The optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
How to handle this antibody:
The antibody solution should be gently mixed before use.
Rabbit anti-mouse IgG (H+L), horseradish Peroxidase (HPR) conjμgated. This antibody has been optimized as secondary antibody in chemiluminescent Western blotting applications
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
anti-Anti-Mouse IgG, -Linked Antibody
Antibody, Mouse, anti, IgG
antibody to-Anti-Mouse Immunoglobulin G, horseradish peroxidase-Linked (Antibody to)