Recombinant Human NPC1 Protein (His & FLAG Tag)

Protein category

Recombinant protein
Source

Human Cells
Target

NPDC1 Protein
Recombinants
Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation

Flag, histidine
Additional source

Recombinants or rec. proteins
Group

recombinants

Gene target

NPC1 Protein His Tag
Gene symbol

NPC1
Short name

Recombinant NPC1 Protein (His & Tag)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. adv advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
Label

His & FLAG tag
Species

Human, Humans
Alternative name

Rec. H. sapiens Niemann-Pick disease, type C1 Protein (histidine & FLAG detection labelled)
Alternative technique

rec
Alternative to gene target

Niemann-Pick disease, type C1, NPC, NPC1 and IDBG-1600 and ENSG00000141458 and 4864, cholesterol binding, nuclei, Npc1 and IDBG-128877 and ENSMUSG00000024413 and 18145, NPC1 and IDBG-643938 and ENSBTAG00000015195 and 286772

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data