FITC (Fluorescein isothiocyanate), Monoclonal Antibody
-
Catalog number
X1542M
-
Price
Please ask
-
Size
100µg
-
-
Host Source
Mouse
-
Clone Name
F4/1
-
Product Category
Ancillary Reagents
-
Species Reactivity
N/A
-
Application
ELISA Western Blot, Flow Cytometry
-
Immunogen
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
-
Purification Method
Protein A/G Chromatography
-
Shipping Conditions
Ship at ambient temperature, freeze upon arrival
-
Storage conditions
FITC (Fluorescein isothiocyanate), Monoclonal Antibody should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
-
-
Properties
If you buy Antibodies supplied by Exalpha they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translation
anticorps
-
Gene target
-
Short name
FITC (Fluorescein isothiocyanate), Monoclonal Antibody
-
Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
-
Isotype
IgG1
-
Label
FITC
-
Alternative name
fluorecein (Fluorescein isothiocyanate), monoclonal (antibody to-)
-
Alternative technique
antibodies, fluorescine
-
MeSH Data
-
Name
-
Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
-
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products