-
Availability
Please contact us to check the availability
-
Immunogen
Synthetic peptide corresponding to the C-terminus of human Synaptophysin
-
Specificity
Synaptophysin, 38 kDa protein in neurectodermal tissue
-
Species reactivity
Human, cattle, mouse, rat
-
Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for min. 20 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. Gentaur Art.No. PU002.
-
Product category
Primary Antibody
-
Antibody s raised in
Rabbit
-
Antibody s isotype
IgG
-
Antibody s clone
SP11
-
Product is supplied as
ready to use
-
Recommended concentration for use
neat
-
Protein concentration
N/A
-
Positive control
Adrenal medulla, brain, pancreas
-
Pretreatment
Pre-treatment with unmasking fluids G (Art. No. DE007) or C (Art. No. DE000).
-
Tested applications
IHC(P)
-
Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable as well as polymeric systems as long as they contain anti-rabbit conjugates.
-
Storage method
Store at 2-8°C
-
UniProt number
PGo to UniProt/SwissProt8247 (SYPH_HUMAN)
-
Litterature
1. Wiedenmann B. and Franke W.W. (1985) Identification and localization of synaptophysin, an integral membrane glykoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41; 1017-1028. 2. Wiedenmann B., Franke W.W., Kuhn C., Moll R., Gould V.E. (1986) Synaptophysin a marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci (USA) 83; 3500-3504. 3. Buffa R., Rindi G., Sessa F., Gini A., Capella C., Jahn R., et al. (1988) Synaptophysin immunoreactivity and small clear vesicles in neuroendocrine cells and related tumours. Moel. Cellul. Probes 2; 367-381. 4. Stefaneanu L., Ryan N, Kovacs K. (1988) Immunocytochemical localization of synaptophysin in human hypophysis and pituitary adenomas. Arch. Pathol. Lab. Med. 112; 801-804
-
Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
