Anti-p53 Antibody

• Catalog number
  F48067-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Anti-p53
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  IHC, WB, ELISA
• Recommended dilutions
  IHC (Paraffin): 1:100,Western blot: 1:1000
• Notes
  Titration of the anti-p53 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This anti-p53 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P04637
• Purity
  Purified
• Description
  Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.
• Immunogen
  A portion of amino acids 364-393 from the human protein was used as the immunogen for this anti-p53 antibody.
• Storage
  Aliquot the anti-p53 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  p53  
• Gene symbol
  MDM4, TP53, LNCPRESS2
• Short name
  Anti-Anti-p53
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Anti-p53
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6974
• Gene
  MDM4
• Long gene name
  MDM4 regulator of p53
• Synonyms gene name
  • mouse double minute 4, human homolog of; p53-binding protein
  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
  • Mdm4 p53 binding protein homolog (mouse)
  • MDM4, p53 regulator
• Synonyms
  • MDMX
  • HDMX
• GenBank acession
  • AF007111
• Locus
  1q32.1
• Discovery year
  1997-09-05
• Entrez gene record
  4194
• Pubmed identfication
  • 9226370
  • 14660608
  • 26798135
• RefSeq identity
  • NM_002393
• Classification
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000035877

Gene info

• Identity
  HGNC:11998
• Gene
  TP53
• Long gene name
  tumor protein p53
• Synonyms
  • p53
  • LFS1
• Synonyms name
  • Li-Fraumeni syndrome
• GenBank acession
  • AF307851
• Locus
  17p13.1
• Discovery year
  1986-01-01
• Entrez gene record
  7157
• Pubmed identfication
  • 6396087
  • 3456488
  • 2047879
• RefSeq identity
  • NM_000546
• VEGA ID
  OTTHUMG00000162125
• Locus Specific Databases
  • IARC TP53 Mutation Database
  • p53 UMD TP53 mutation database
  • Database of Germline p53 Mutations
  • MUTP53LOAD, Mutant p53 Loss Of Activity Database
  • LRG_321

Gene info

• Identity
  HGNC:52747
• Gene
  LNCPRESS2
• Long gene name
  lncRNA p53 regulated and ESC associated 2
• Synonyms name
  • lncRNA p53 regulated and ESC specific 2
• Locus
  4q22.1
• Discovery year
  2016-12-14
• Entrez gene record
  101929194
• Pubmed identfication
  • 27912097
• RefSeq identity
  • NR_125920
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000160917

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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