DGKA Colorimetric Cell-Based ELISA
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Catalog number
CB5861
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Price
Please ask
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Size
One 96-Well Plate
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Description
DGKA Colorimetric Cell-Based ELISA is an appropriate, lysatefree, high throughput and high sensitivity assay kit that can check DGKAprotein and its expression profile in cells. This kit can be used in order to measur with a hight precision the relative amounts of DGKAinsid the cultured cells as well as detection of the effects that various inhibitors or treatments (ie. siRNA or chemicals), or activators have on DGKA.
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Target Name
DGKA
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Assay Type
Non-Phospho Cell-Based ELISA
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Detection Method
Colorimetric 450 nm
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Synonyms
DGKA; DAGK; DAGK1 ; DGK-alpha; 80 kDa diacylglycerol kinase ; DAG kinase alpha; Diglyceride kinase alpha ; Diacylglycerol kinase alpha.
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NCBI Gene Symbol
DGKA
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Storage
4°C
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Stability
6 Months
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Dynamic Range
> 5000 Cells
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Recommended use
Research use only
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Contact us
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Technical Service
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
DGKA
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Short name
DGKA Colorimetric -Based ELISA
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Technique
colorimetric, ELISA, colorimetry, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
diacylglycerol kinase, alpha 80kDa colorimetry cellular-Based Enzyme-linked immunosorbent assay
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Alternative technique
assays, elisas
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Alternative to gene target
diacylglycerol kinase, alpha 80kDa, DAGK and DAGK1 and DGK-alpha, DGKA and IDBG-39397 and ENSG00000065357 and 1606, phospholipid binding, Plasma membranes, Dgka and IDBG-198283 and ENSMUSG00000025357 and 13139, DGKA and IDBG-636422 and ENSBTAG00000004018 and 506348
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Tissue
cell
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Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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