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Rabbit Anti Methylated Lysine Polyclonal

Rabbit Anti Methylated Lysine Polyclonal is available 19 times from Stressmark antibodies labs

SPC-158F-ALP | Rabbit Anti-Methylated Lysine Polyclonal: Alkaline Phosphatase size: 400 µl | 441.24 USD

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SPC-158F-APC | Rabbit Anti-Methylated Lysine Polyclonal: APC size: 400 µl | 446.76 USD

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SPC-158F-A390 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 390 size: 400 µl | 448.96 USD

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SPC-158F-A488 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 488 size: 400 µl | 447.86 USD

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SPC-158F-A565 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 565 size: 400 µl | 447.86 USD

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SPC-158F-A594 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 594 size: 400 µl | 447.86 USD

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SPC-158F-A633 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 633 size: 400 µl | 447.86 USD

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SPC-158F-A655 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 655 size: 400 µl | 447.86 USD

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SPC-158F-A680 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 680 size: 400 µl | 447.86 USD

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SPC-158F-A700 | Rabbit Anti-Methylated Lysine Polyclonal: ATTO 700 size: 400 µl | 447.86 USD

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SPC-159F | Rabbit Anti-Methylated Lysine Polyclonal: Biotin size: 400 µl | 441.24 USD

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SPC-158F-FITC | Rabbit Anti-Methylated Lysine Polyclonal: FITC size: 400 µl | 439.03 USD

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SPC-160F | Rabbit Anti-Methylated Lysine Polyclonal: HRP size: 400 µl | 458.89 USD

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SPC-158F-P594 | Rabbit Anti-Methylated Lysine Polyclonal: PE/ATTO 594 size: 400 µl | 455.58 USD

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SPC-158F-PCP | Rabbit Anti-Methylated Lysine Polyclonal: PerCP size: 400 µl | 446.76 USD

Catalog number SPC-158F-PCP
Price 446.76 USD
Size 400 µl
Certificate of analysis 0.2-0.5 µg/ml of SPC-158 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
Net weight (g) 0.4
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Conjugate Peridinin chlorophyll (PerCP)
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetMethylated Lysine
Short name Rabbit Anti-Methylated Lysine Polyclonal: PerCP
Technique Polyclonal, Rabbit, Percp, anti
Label PerCP
Alternative name production species: rabbit antibody to-Methylated Lysine polyclonal: PerCP
MeSH Data
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200

SPC-158F-RPE | Rabbit Anti-Methylated Lysine Polyclonal: RPE size: 400 µl | 444.55 USD

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SPC-158F-STR | Rabbit Anti-Methylated Lysine Polyclonal: Streptavidin size: 400 µl | 445.65 USD

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SPC-158F | Rabbit Anti-Methylated Lysine Polyclonal: Unconjugated size: 400 µl | 396.01 USD

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SPC-158S | Rabbit Anti-Methylated Lysine Polyclonal: Unconjugated size: 12 µl | 55.16 USD

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PubMed number 27858264|27521690|25609799|25374779|24651940|24233354|23533717
Recommended buffer for storage PBS, 50% glycerol
Antibody's reactivity Species Independent
Warning information Non-hazardous
Representative figure legend Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite. | Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right). Rabbit Anti-Methylated Lysine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-158) | Rabbit Anti-Methylated Lysine Antibody used in Western blot (WB) on Bovine serum albumin (SPC-158)
Raised in Rabbit
NCBI number Refer to NCBI
Gene number Refer to GenBank
Released date 1/Jun/2007
Immunogen Methylated KLH Conjugated
Antibody's other name Dimethylysine Antibody, Methyl lysine Antibody, N epsilon dimethyl lysine Antibody, Trimethyl lysine Antibody
Protein number Refer to Swiss-Prot
Antibody's recommended dilutions for use WB (1:5000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Tested applications Dot Blot|Western Blot|Western Blot|Western Blot|Dot Blot|Dot Blot|Dot Blot
Primary research fields Cell Signaling, Post-Translational Modifications, Methylation
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Total weight (kg) 1.4
Stock availabilit In Stock
Scientific context Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
Antibody's target Methylated Lysine
Tissue specificity See included datasheet or contact our support service.
Antibody's full description Rabbit Anti-Methylated Lysine Polyclonal
Country of production Canada
Antibody's purified from Protein A Purified
Antibody's concentration 0.25 mg/ml
Tested reactivity Otala lactea (milk snail)|Squirrel|Hamster |Ictidomys tridecemlineatus (ground squirrel)|Xenopus laevis|Littorinalittorea (intertidal marine snail)|Turtle
Antibody in cell Contact our support service.
Antibody's category Polyclonal Antibodies
Representative figure linkmore | details
Bibliography 1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
Verified applications WB, ICC/IF, IP, ELISA
Antibody's specificity Detects proteins containing methylated lysine residues.
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Host Rabbit
Alternative technique polyclonals
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Rabbit Anti-Methylated Lysine Polyclonal: PerCP
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  • Methylated
  • Lysine
  • Alkaline
  • Phosphatase
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