Clonality Monoclonal Antibody (Clone#4B3-G7-H5)
Description Mouse Monoclonal antibody to detect EEF2
Physical state Colorless liquid
Purity Affinity purified
Raised in Mouse
Gene number 1938
Target EEF2 (EEF2, Eef2, EF-2, EF2, EF2_HUMAN, Elongation factor 2, Eukaryotic translation elongation factor 2, Polypeptidyl tRNA translocase, SCA26)
Package form Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Immunogen Recombinant human EEF2 protein fragments expressed in E.coli
Protein number P13639
Precautions Manifactured in USA. For Research Use Only! Not to be used in humans
Released This antibody is available to order since May 2017
Scientific context Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Approved applications with antibody dilution Western blot; 1:5000, Immunofluorescence; 1:200
Handling and storage Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Antibody's positive controles Western blot: HL-60, Jurkat, SHSY-5Y, U20S and Hela cell lysates; Immunofluorescence: HeLa cells
Reacts with Human
Research area Epigenetics
Delivery Shipped to your lab under gel pack
Additional description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus
French translation anticorps