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Synonyms
CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34
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Alternative_names
CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34
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Description
Plays a critical role in MHC class II antigen processing
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 ug/ml (100 ul/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 ug/ml (100 ul/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.
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Molecular Weight
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 19.3 kDa. The predicted N-terminus is Gln 73. DTT-reduced Protein migrates as 23-33 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Cluster of Differentiation 74 (CD74) also known as HLA class II histocompatibility antigen gamma chain (HLADG), is a polypeptide involved in the formation and transport of MHC class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD74
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Short name
CellExp™ CD74/DHLAG, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ CD74 molecule, major histocompatibility complex, class II invariant chain/DHLAG, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAG and HLADG and Ia-GAMMA and II, CD74 and IDBG-53594 and ENSG00000019582 and 972, MHC class II protein binding, Cell surfaces, Cd74 and IDBG-150302 and ENSMUSG00000024610 and 16149, BT.46809 and IDBG-646288 and ENSBTAG00000015228 and 613384
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