Mouse Anti-Mouse BK Beta2 Monoclonal IgG1, Clone S53-32
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Catalog numberSMC-331D-HRP
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PricePlease ask
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Size100 µg
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CloneS53-32
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ImmunogenFusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2
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Antibody s full descriptionMouse Anti-Mouse BK Beta2 Monoclonal IgG1 Antibody, Clone: S53-32: HRP
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameKCNMB2 Antibody, Calcium-activated potassium channel subunit beta-2 Antibody, BK channel subunit beta-2 Antibody, BKbeta2 Antibody, Calcium-activated potassium channel Antibody, subfamily M subunit beta-2 Antibody, Charybdotoxin receptor subunit beta-2 Antibody, K(VCA)beta-2 Antibody, Maxi K channel subunit beta-2 Antibody, Slo-beta-2 Antibody
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Raised inMouse
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Antibody s targetBK Beta2
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Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Calcium-Activated Potassium Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF, IP, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~27kDa. No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-331 was sufficient for detection of BK Channel beta2 in 10 µg of COS-1 cells transiently transfected with KBeta2 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextBK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (1). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (2). This has major implications on other physiological processes in tissues other than the brain.
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Bibliography1. Wulf-Johansson H., et al. (2009) Brain Res. 1292: 1-13. 2. Lee U.S., and Cui J. (2009) J Physiol. 587(7): 1481-1489.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_082507.1
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Gene number72413
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Protein numberQ9CZM9
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunohistochemistry analysis using Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody, Clone S53-32 (SMC-331). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody (SMC-331) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower epidermal cells positive, but quite dull. Western Blot analysis of Human Cell line lysates showing detection of BK Beta2 Potassium Channel protein using Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody, Clone S53-32 (SMC-331). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody (SMC-331) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody, Clone S53-32 (SMC-331). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody (SMC-331) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dottiness and dull. Mouse Anti-BK Beta2 Potassium Channel Antibody [S53-32] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-331) Mouse Anti-BK Beta2 Potassium Channel Antibody [S53-32] used in Western Blot (WB) on Human Cell line lysates (SMC-331) Mouse Anti-BK Beta2 Potassium Channel Antibody [S53-32] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-331)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGKV1D-32, SNORD115-32, PPP1R1B, IGLV3-32, IGHV3-32, IGKV1-32, CUTC, ZNF138, LINC01234, LUARIS
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Short nameMouse Anti-Mouse BK Beta2 Monoclonal IgG1, Clone S53-32
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelHRP
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse BK Beta2 monoclonal IgG1, clonality S53-32
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Alternative techniquemurine, antibodies
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Clone nameS53-32
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-32
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-32
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory inhibitor subunit 1B
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 1B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-32 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 3-32
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-32 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-32
- immunoglobulin heavy variable 3-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1-32
- immunoglobulin kappa variable 1-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecutC copper transporter
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Synonyms gene name
- cutC copper transporter homolog (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 138
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Synonyms gene name
- zinc finger protein 138 (clone pHZ-32)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-02-11
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Entrez gene record
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1234
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Synonyms
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Locus
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Discovery year2014-02-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelncRNA upregulator of antiviral response interferon signaling
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Synonyms
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Synonyms name
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Locus
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Discovery year2017-03-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
