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Immunogen
Peptide derived from N-terminal region of human LC3A protein
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Antibody s target
Human LC3A
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Antibody s full description
Rabbit Anti-Human LC3A Polyclonal
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Primary research fields
Cancer, Autophagy, Cell Signaling, Neuroscience, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
MAP1LC3A Antibody, ATG8F Antibody, Autophagy related protein LC3 A Antibody, Autophagy related ubiquitin like modifier LC3 A Antibody, MAP1LC3A Antibody, Microtubule associated protein 1 light chain 3 alpha Antibody, LC3 A Antibody, LC3 Antibody, MAP1 light chain 3 like protein 1 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B LC3 A Antibody, MAP1ALC3 Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Salt precipitated
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Recommended buffer for storage
PBS, 0.09% sodium azide
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Antibody s concentration
0.25 mg/ml
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Antibody s specificity
Specific for LC3A. Does not detect LC3B, LC3C, GABARAP, GABARAPL1 or GABARAPL2. Detects LC3A-I and LC3A-II.
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:5000 dilution of SPC-216 was sufficient for detection of LC3A in lysate from cells expressing recombinant GST-tagged LC3A by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cytoskeleton, Endomembrane System, Lipid-anchor, Cytoplasmic Vesicle, Autophagosome, Autophagosome Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
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Bibliography
1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
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Released date
12/Jun/2014
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NCBI number
NP_852610.9
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Gene number
84565
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Protein number
Q9H500
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.05
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
