Human CellExp™CNTFR/CNTFR-alpha, human recombinant
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Catalog number7836-50
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PricePlease ask
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Size50 ug
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SynonymsCNTFR, CNTF R alpha
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Alternative_namesCNTFR, CNTF R alpha
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DescriptionThis receptor and its cognate ligand support the survival of neurons.
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RecombinantYes
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SourceHEK 293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Endotoxin Level< 1.0 EU per ug of the rh CNTFR alpha by the LAL method.
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Activity Specifications test methodMeasured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 ug/ml (100 ul/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 ug/ml (100 ul/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
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ResultsMeasured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 ug/ml (100 ul/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
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Molecular WeightThis protein rh CNTFR alpha is fused with polyhistidine tag at the C-terminus, has a calculated MW of 36.9 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 45-48 kDa due to glycosylation.
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
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Background InformationCiliary neurotrophic factor receptor subunit alpha (CNTFR or CNTFR-alpha) belongs to the type I cytokine receptor family and type 3 subfamily. CNTFR contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. This receptor and its cognate ligand support the survival of neurons. This receptor is most closely related to the interleukin-6 receptor. This receptor possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionThe CellExp™CNTFR/CNTFR-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCNTFR, CNTFR-AS1
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Short nameCellExp™CNTFR/CNTFR-alpha, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ciliary neurotrophic factor receptor/ciliary neurotrophic factor receptor-a, H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetciliary neurotrophic factor receptor, CNTFR and IDBG-59992 and ENSG00000122756 and 1271, cytokine binding, Plasma membranes, Cntfr and IDBG-138110 and ENSMUSG00000028444 and 12804, BT.46623 and IDBG-635903 and ENSBTAG00000015361 and 539548
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Gene info
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Identity
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Gene
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Long gene nameciliary neurotrophic factor receptor
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GenBank acession
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Locus
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Discovery year1992-09-22
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Entrez gene record
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Pubmed identfication
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Classification
- Fibronectin type III domain containing
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCNTFR antisense RNA 1
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GenBank acession
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Locus
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Discovery year2013-06-07
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Entrez gene record
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Classification
- Antisense RNAs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data