Anti- Leptin (LEP)-Mouse monoclonal Antibody
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Catalog number
MAA084Ra21
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Price
Please ask
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Size
10ug
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Organism Species
Rattus norvegicus (Rat)
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Source
Monoclonal antibody preparation
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Purification
Protein A + Protein G affinity chromatography
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Buffer Formulation
PBS, pH7.4,containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Item Name
Leptin
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Immunogen
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Image number
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Species reactivity
Rat
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Sequence of immunogen
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Aplication
WB; IHC; ICC; IP.
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Clonality
Mouse monoclonal
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Concentration
1mg/ml
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Alternative Names
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
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Applicable Secondary Antibody
SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
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Manual link
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Delivery condition
4℃ with ice bags
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Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
LEP
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Short name
Anti- Leptin (LEP)-Mouse monoclonal Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, anti, antibody to, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Mouse, Mouses
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Alternative name
antibody to- Leptin (leptin)-Mouse monoclonal (antibody to-)
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Alternative technique
antibodies, murine
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Alternative to gene target
leptin, LEPD and OB and OBS, LEP and IDBG-39587 and ENSG00000174697 and 3952, peptide hormone receptor binding, Extracellular, Lep and IDBG-130781 and ENSMUSG00000059201 and 16846, OBESE and IDBG-635003 and ENSBTAG00000014911 and 280836
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Gene info
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Identity
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Gene
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Long gene name
leptin
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Synonyms gene
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Synonyms gene name
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
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Locus
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Discovery year
1993-01-26
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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