mCherry Fluorescent Protein
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Catalog number
4993-5000
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Price
Please ask
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Size
5 mg
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Synonyms
mCherry
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Alternative_names
mCherry
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Description
Fluorescent protein ideal for subcellular labeling/visualization
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Purity
≥97%
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Molecular Weight
28.8 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Freeze Dried
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Reconstitution Instructions
Reconstitute with dH₂O to 1 mg/ml
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Background Information
mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. The protein is a 28.8 kDa monomer with 256 amino acids, pI: 6.23. Ex.= 587 nm (540-590 nm); Em.= 610 nm (550-650 nm). The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody (BioVision Cat # 3998-100) or protein purification/removal by using Ni++ beads.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional description
Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
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