mCherry Fluorescent Protein

  • Catalog number
    4993-5000
  • Price
    Please ask
  • Size
    5 mg
  • Synonyms
    mCherry
  • Alternative_names
    mCherry
  • Description
    Fluorescent protein ideal for subcellular labeling/visualization
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥97%
  • Assay
    SDS-PAGE
  • Purity
    ≥97%
  • Molecular Weight
    28.8 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Freeze Dried
  • Reconstitution Instructions
    Reconstitute with dH₂O to 1 mg/ml
  • Background Information
    mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. The protein is a 28.8 kDa monomer with 256 amino acids, pI: 6.23. Ex.= 587 nm (540-590 nm); Em.= 610 nm (550-650 nm). The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody (BioVision Cat # 3998-100) or protein purification/removal by using Ni++ beads.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Additional description
    Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
  • Gene target
  • Short name
    mCherry Protein
  • Label
    fluorescent
  • Alternative name
    mCherry Fluorescent Protein
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