Description NZM Growth Agar is used for lambda and filamentous phage. To prepre the media d issolve the powder in d istilled water, heat to boiling if it is necessary, then d ispense as desired and sterilize by autoclaving at 121°C for 15 minutes.
Appearance of powder Cream to yellow coloured, homogeneous, free flowing powder.
Quantity required by litre 30 g
Organisms (ATCC) Growth Escherichia coli: good-luxuriant
Colour and Clarity Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Composition (g/l) Casein enzymic hydrolysate 10.00 MgSO4. 7H2O 2.00 Sodium chloride 5.00 Agar 13.00 Final pH (at 25oC) 7.0 ± 0.2
Cultural Response Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Storage Store below 30°C and the prepared medium at 2 - 8°C.
Application Molecular Biology Growth Media for Escherichia coli