NMNAT2, human recombinant

• Catalog number
  7811-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Nicotinamide mononucleotide Adenylyltransferase 2, C1orf15, PNAT2
• Alternative_names
  Nicotinamide mononucleotide Adenylyltransferase 2, C1orf15, PNAT2
• Description
  Catalyzes an essential step in NAD (NADP) biosynthetic pathway
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥85%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity: > 500 pmol/min/µg. One unit will convert 1.0 pmole of beta-NADH per minute to beta-NAD at PH 8.0 at 37ºC
• Results
  Specific activity: > 500 pmol/min/µg
• Unit Definition
  One unit will convert 1.0 pmole of beta-NADH per minute to beta-NAD at PH 8.0 at 37ºC
• Molecular Weight
  36.6 kDa (327 aa, 1-307 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.25 mg/ml
• Appearance
  Liquid
• Physical form description
  0.25 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 150 mM NaCl and 1 mM DTT.
• Background Information
  NMNAT2 belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human NMNAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQN SNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ LYINASG
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NMNAT2  
• Gene symbol
  NMNAT2
• Short name
  NMNAT2, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  NMNAT2, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:16789
• Gene
  NMNAT2
• Long gene name
  nicotinamide nucleotide adenylyltransferase 2
• Synonyms gene
  • C1orf15
• Synonyms gene name
  • chromosome 1 open reading frame 15
• Synonyms
  • KIAA0479
  • PNAT2
• GenBank acession
  • AF288395
• Locus
  1q25.3
• Discovery year
  2001-10-08
• Entrez gene record
  23057
• Pubmed identfication
  • 11318611
  • 12359228
• VEGA ID
  OTTHUMG00000035519

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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