anti-LEFTY2 Antibody [7C5G1H6H10]
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Catalog number
51-035
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Price
Please ask
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Size
0.05 mg
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Gene info
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-β family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-β (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-β actions are directed by ligand-induced activation of TGF-β receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-β superfamily, inhibits TGF-β signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-β receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-β-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-β superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 kDa) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.
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Gene names
LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4
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Antibodie s label
unlabelled serum
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Purity
Protein A Column
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
[7C5G1H6H10]
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Immunoglobulin
IgG2a,k
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Spicific to
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
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Antigen
LEFTY2 monoclonal antibody was made against recombinant LEFTY2 (Human). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry
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Antibody advice
LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 ug/mL.
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Suspention pH azide
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
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Keep at
Store LEFTY2 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Human
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Omim nr
13124811
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Ncbi nr
O00292
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Vial with antibody
in solution
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NCBI GENE nr
7044
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GENE symbol
LEFTY2
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Antigene name
left-right determination factor 2
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Source
Homo sapiens
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Protein nr
O00292
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
LEFTY2
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Short name
anti-LEFTY2 Antibody [7C5G1H6H10]
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toleft-right determination factor 2 (antibody to-) [7C5G1H6H10]
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Alternative technique
antibodies
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Alternative to gene target
left-right determination factor 2, EBAF and LEFTA and LEFTYA and TGFB4, LEFTY2 and IDBG-106991 and ENSG00000143768 and 7044, growth factor activity, Extracellular
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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