Recombinant Stem Cell Factor (SCF)

• Catalog number
  RPU43890-1mg
• Price
  Please ask
• Size
  1mg

• Estimated Turnaround Time
  6-11 business days
• Gene Name
  NA
• Alternative Names
  KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2
• Source
  E.coli
• Purity
  ≥ 95%
• Application
  Positive Control; Immunogen; SDS-PAGE; WB.
• Shipping Conditions
  Ice packs
• Storage conditions
  Short term: -20°C; Long term: -80°C. Minimize freeze and thaw cycles.
• Shelf life
  1 year
• Restriction
  For research use only. Not for diagnostic procedures.
• Description
  For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants
• Test
  Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

• Gene target
  Stem   Factor   SCF  
• Gene symbol
  KITLG
• Short name
  Recombinant Stem Factor (SCF)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biomatik advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Rattus norvegicus (Rat)
• Alternative name
  Rec. progenitor cellular Factor (SCF)
• Alternative technique
  rec
• Tissue
  cell, stem

Gene info

• Identity
  HGNC:6343
• Gene
  KITLG
• Long gene name
  KIT ligand
• Synonyms gene
  • MGF
• Synonyms
  • SCF
  • SF
  • Kitl
  • KL-1
  • FPH2
  • SLF
  • DFNA69
• Synonyms name
  • mast cell growth factor
  • stem cell factor
  • steel factor
  • familial progressive hyperpigmentation 2
• GenBank acession
  • M59964
• Locus
  12q21.32
• Discovery year
  1991-06-04
• Entrez gene record
  4254
• Pubmed identfication
  • 2208279
  • 1707188
  • 19375057
  • 26522471
• RefSeq identity
  • NM_003994
• VEGA ID
  OTTHUMG00000169888
• Locus Specific Databases
  • LRG_1379

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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