Epigen (EPGN), human recombinant

• Catalog number
  4969-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
• Alternative_names
  EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
• Description
  Promotes the growth of epithelial cells
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expected ED₅₀ for this effect is 150-300 ng/ml.
• Results
  150-300 ng/ml
• Molecular Weight
  7.9 kDa
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Sterile filtered and lyophilized from 20 mM PBS buffer pH-7.4 containing 130 mM sodium chloride
• Reconstitution Instructions
  Reconstitute in sterile dH₂O not less than 100 ug/ml.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
• Background Information
  EPGN is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human epigen produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Epigen   EPGN  
• Gene symbol
  EPGN
• Short name
  Epigen (EPGN), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Epigen (EPGN), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:17470
• Gene
  EPGN
• Long gene name
  epithelial mitogen
• Synonyms gene name
  • epithelial mitogen homolog (mouse)
• Synonyms
  • EPG
  • PRO9904
  • ALGV3072
• Synonyms name
  • epigen
• Locus
  4q13.3
• Discovery year
  2006-10-17
• Entrez gene record
  255324
• RefSeq identity
  • NM_001013442
• VEGA ID
  OTTHUMG00000160869

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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