NADP/NADPH Quantitation Colorimetric Kit

Description

No NAD or NADH Detection
Summary

• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications- Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio
Sample Type

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features Benefits

• Simple procedure; takes ~2 hours • Fast and convenient • Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio • Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH)
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NADᶧ/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.
Data Sheet Link
Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Gene target

NADP/NADPH Quantitation Kit
Short name

NADP/NADPH Quantitation Colorimetric Kit
Technique

colorimetric, colorimetry
Alternative name

NADP/NADPH Quantitation colorimetry reagent
Alternative technique

assays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data