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Category
Negative Control
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Long description
This monoclonal antibody to IgG1 is a negative control for Immunofluorescence staining with Pure, FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. It is used to monitor the level of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.
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Antibody come from
n/a
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
ZX3
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Antigen antibody binding interaction
Mouse IgG1 antibody conjugated to FITC - Negative Control
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Antibody is raised in
Mouse
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Flow Cytometry
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Antibody s suited for
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.
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Storage
4ºC
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Relevant references
1. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. _x000B__x000B_2. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. _x000B__x000B_Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
